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KMID : 0616120200510010078
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Korean Journal of Pharmacognosy
2020 Volume.51 No. 1 p.78 ~ p.85
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HPLC Method Validation for Quantitative Analysis of Scopoletin from Hot-Water Extract Powder of Artemisia annua Linne
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Kim Seon-Hee
Yoon Kee-Dong
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Abstract
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In this study, we shortly introduced the HPLC method validation guideline for the analysis of functional food which was released from the Ministry of Food and Drug Safety of Korea in Dec 2018. The HPLC method validation was performed through the aforementioned HPLC method validation guideline in order to quantitate scopoletin content from the hot-water extract powder of Artemisia annua Linne. The HPLC method was validated by evaluating specificity, accuracy, precision, limit of quantitation and linearity. All parameters were in the suitable ranges which are designated in the guideline, which indicated the current HPLC method is reliable to quantitate the scopoletin content from the hot-water extract of A. annua.
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KEYWORD
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Artemisia annua, Hot-water extract, Scopoletin, HPLC method validation guideline
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